The use of new tools for the study of genetic of marine populations is important for the development and implementation of genetic improvement programs. The use of DNA markers in marine population has made little progress compared with their use in terrestrial species. DNA markers can be used to verify pedigrees as well as to screen wild populations to maximise diversity in founder animals. These knowledges can be applied to many aspects such as stock management, creation of reserves, etc. In particular they can be applied to commercially important species such as Dicentrarchus labrax that is an important farmed specie in Europe. The information available on population genetic structure of sea bass suggests that there are significant population subdivision in both Atlantic and Mediterranean parts of its range. Further work in this area will benefit from the availability of useful molecular markers. AFLP markers provide an efficient method for DNA fingerprinting in many species of plants and animals. In this work new AFLP markers were generated for sea bass (Dicentrarchus labrax). Two main factors affect the number of AFLP bands generated per individual: 1) the specific endonucleases used and 2) the number of selective bases added to the primers in the selective amplification step. The AFLP markers utilised in this study were generated by EcoRI and MseI restriction and by using primers with three selective bases. These AFLP markers were used to start an investigation on population structure of sea bass in southern Italy by comparing the animals captured in two different geographic areas in Puglia: Acquatina lagoon and Alimini lagoon.
Development of AFLP markers to determine genetic diversity in population of sea bass Dicentrarchus labrax in two areas of mediterranean sea
ZILLI, Loredana;PERROTTA, Carla;STORELLI, Carlo;VILELLA, Sebastiano
2004-01-01
Abstract
The use of new tools for the study of genetic of marine populations is important for the development and implementation of genetic improvement programs. The use of DNA markers in marine population has made little progress compared with their use in terrestrial species. DNA markers can be used to verify pedigrees as well as to screen wild populations to maximise diversity in founder animals. These knowledges can be applied to many aspects such as stock management, creation of reserves, etc. In particular they can be applied to commercially important species such as Dicentrarchus labrax that is an important farmed specie in Europe. The information available on population genetic structure of sea bass suggests that there are significant population subdivision in both Atlantic and Mediterranean parts of its range. Further work in this area will benefit from the availability of useful molecular markers. AFLP markers provide an efficient method for DNA fingerprinting in many species of plants and animals. In this work new AFLP markers were generated for sea bass (Dicentrarchus labrax). Two main factors affect the number of AFLP bands generated per individual: 1) the specific endonucleases used and 2) the number of selective bases added to the primers in the selective amplification step. The AFLP markers utilised in this study were generated by EcoRI and MseI restriction and by using primers with three selective bases. These AFLP markers were used to start an investigation on population structure of sea bass in southern Italy by comparing the animals captured in two different geographic areas in Puglia: Acquatina lagoon and Alimini lagoon.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
