A potentiometric urea biosensor based on urease (Ur) electrochemical immobilisation by poly( o -phenylenediamine) (PPD) is proposed. Polymer films have been grown by cyclic voltammetry on a glassy carbon (GC) electrode, using an unconventional “upside-down” (UD) geometry. GC/Ur-PPD electrodes exhibit a rapid (5–10 s) and sensitive response to urea concentration and lifetime of at least 5 weeks. Work is in progress to optimise the sensor and study its behaviour in the presence of possible interferences.

A New Potentiometric Urea Biosensor Based on Urease Immobilized in Electrosyntesised Poly(o-Phenylenediamine)

CHIRIZZI, DANIELA;MALITESTA, Cosimino
2011-01-01

Abstract

A potentiometric urea biosensor based on urease (Ur) electrochemical immobilisation by poly( o -phenylenediamine) (PPD) is proposed. Polymer films have been grown by cyclic voltammetry on a glassy carbon (GC) electrode, using an unconventional “upside-down” (UD) geometry. GC/Ur-PPD electrodes exhibit a rapid (5–10 s) and sensitive response to urea concentration and lifetime of at least 5 weeks. Work is in progress to optimise the sensor and study its behaviour in the presence of possible interferences.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11587/366486
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