A potentiometric urea biosensor based on urease (Ur) electrochemical immobilisation by poly( o -phenylenediamine) (PPD) is proposed. Polymer films have been grown by cyclic voltammetry on a glassy carbon (GC) electrode, using an unconventional “upside-down” (UD) geometry. GC/Ur-PPD electrodes exhibit a rapid (5–10 s) and sensitive response to urea concentration and lifetime of at least 5 weeks. Work is in progress to optimise the sensor and study its behaviour in the presence of possible interferences.
A New Potentiometric Urea Biosensor Based on Urease Immobilized in Electrosyntesised Poly(o-Phenylenediamine)
CHIRIZZI, DANIELA;MALITESTA, Cosimino
2011-01-01
Abstract
A potentiometric urea biosensor based on urease (Ur) electrochemical immobilisation by poly( o -phenylenediamine) (PPD) is proposed. Polymer films have been grown by cyclic voltammetry on a glassy carbon (GC) electrode, using an unconventional “upside-down” (UD) geometry. GC/Ur-PPD electrodes exhibit a rapid (5–10 s) and sensitive response to urea concentration and lifetime of at least 5 weeks. Work is in progress to optimise the sensor and study its behaviour in the presence of possible interferences.File in questo prodotto:
Non ci sono file associati a questo prodotto.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.