Aims: To study the importance of IkBa in NF-kB signal transduction, we analysed the IkBa expression in monocytes from Sjo¨gren’s syndrome (SS) patients versus healthy controls. Methods: Monocytes were obtained from the peripheral blood of 30SSpatients and 23 healthy subjects. IkBaexpressionwas studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA). Results: Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IkBa, and in all the Sjo¨gren’s syndrome cases examined, serum IkBa levels were significantly decreased in comparison with controls. Conclusions: Our findings clearly demonstrate changes in the levels of IkBa in SS monocytes, suggesting that the attenuated expression of IkBa could contribute to the deregulation of NF-kB pathways in the SS pathogenesis. Decreased expression of IkBa may specifically amplify cytokines production and inflammatory response linked to Sjo¨gren’s syndrome.

Altered IkBa expression promotes NF-kB activation in monocytes fromprimary Sjogren’s syndrome patients

LOFRUMENTO, Dario Domenico
Penultimo
Membro del Collaboration Group
;
2012-01-01

Abstract

Aims: To study the importance of IkBa in NF-kB signal transduction, we analysed the IkBa expression in monocytes from Sjo¨gren’s syndrome (SS) patients versus healthy controls. Methods: Monocytes were obtained from the peripheral blood of 30SSpatients and 23 healthy subjects. IkBaexpressionwas studied by semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR), real-time PCR, immunoblotting, flow cytometry and enzyme linked immunosorbent assay (ELISA). Results: Analysis of the gene and protein expression profiles of SS monocytes revealed a down-regulation of IkBa, and in all the Sjo¨gren’s syndrome cases examined, serum IkBa levels were significantly decreased in comparison with controls. Conclusions: Our findings clearly demonstrate changes in the levels of IkBa in SS monocytes, suggesting that the attenuated expression of IkBa could contribute to the deregulation of NF-kB pathways in the SS pathogenesis. Decreased expression of IkBa may specifically amplify cytokines production and inflammatory response linked to Sjo¨gren’s syndrome.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11587/371480
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