Sorghum is source of antioxidant compounds including phenolic acids, flavonoids and condensed tannins. In this study, we measured the antioxidant capacity of seven white food-grade sorghum hybrids grown in Southern Italy using the Ty1antiROS cell-based test and the chemical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) assays. Samples were also analyzed for phenolic contents. Ty1antiROS test showed that all hybrids possessed antioxidant activity and were effective in scavenging the ABTS•+ target species but they exerted a weak DPPH• antiradical effect. Furthermore, all samples had similar values for total phenolic content. Statistical analyses showed a positive correlation between the ABTS and DPPH data (R = 0.61) and a weakly positive correlation between the ABTS and Ty1antiROS data (R= 0.38). The utilization of both biological and chemical tests for the antioxidant capacity evaluation make us able to select varieties of sorghum with high antioxidant potential useful to promote human health.
Measurement of biological antioxidant activity of seven food-grade sorghum hybrids grown in a Mediterranean environmen
Pizzolante, Graziano;Alifano, Pietro;
2016-01-01
Abstract
Sorghum is source of antioxidant compounds including phenolic acids, flavonoids and condensed tannins. In this study, we measured the antioxidant capacity of seven white food-grade sorghum hybrids grown in Southern Italy using the Ty1antiROS cell-based test and the chemical 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) assays. Samples were also analyzed for phenolic contents. Ty1antiROS test showed that all hybrids possessed antioxidant activity and were effective in scavenging the ABTS•+ target species but they exerted a weak DPPH• antiradical effect. Furthermore, all samples had similar values for total phenolic content. Statistical analyses showed a positive correlation between the ABTS and DPPH data (R = 0.61) and a weakly positive correlation between the ABTS and Ty1antiROS data (R= 0.38). The utilization of both biological and chemical tests for the antioxidant capacity evaluation make us able to select varieties of sorghum with high antioxidant potential useful to promote human health.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
