Differential effects of high-carbohydrate and high-fat diets on hepatic lipogenesis in rats

PURPOSE: Hepatic fatty acid synthesis is influenced by several nutritional and hormonal factors. In this study, we have investigated the effects of distinct experimental diets enriched in carbohydrate or in fat on hepatic lipogenesis. METHODS: Male Wistar rats were divided into four groups and fed distinct experimental diets enriched in carbohydrates (70% w/w) or in fat (20% and 35% w/w). Activity and expression of the mitochondrial citrate carrier and of the cytosolic enzymes acetyl-CoA carboxylase and fatty acid synthetase were analyzed through the study with assessments at 0, 1, 2, 4 and 6 weeks. Liver lipids and plasma levels of lipids, glucose and insulin were assayed in parallel. RESULTS: Whereas the high carbohydrate diet moderately stimulated hepatic lipogenesis, a strong inhibition of this anabolic pathway was found in animals fed high fat diets. This inhibition was time and concentration-dependent. Moreover, whereas the high carbohydrate diet induced an increase in plasma triglycerides, the high fat diets determined an accumulation of triglycerides in liver. An increase in the plasmatic levels of glucose and insulin was observed in all cases. CONCLUSIONS: The excess of sucrose in the diet is converted into fat, which is distributed by bloodstream in the organism in the form of circulating triglycerides. On the other hand, a high amount of dietary fat caused a strong inhibition of lipogenesis and a concomitant increase in the level of hepatic lipids, thereby highlighting, in these conditions, the role of liver as a reservoir of exogenous fat.